PhysioSuite FAQs
Are the MouseSTAT Pulse Oximeter Sensors CT compatible?
Yes, all MouseSTAT sensors are CT compatible.
Can I output data?
Yes, there are multiple options to record and manage data. The system includes one analog output (for a single parameter), or you may export all parameters digitally via mini-USB.
Can the MouseSTAT measure SpO2 on 7-day-old mice?
Yes, you can use the 4 mm paw sensor or try using the Y-Clip sensor as a whole body sensor.
Can the MouseSTAT Pulse Oximeter be used on awake animals?
Scientists in search of equipment to meet their study needs should be aware that there are legitimate questions regarding even the possibility of taking these measurements in mobile animals. In reality, there is currently no system capable of 100% reliably measuring pulse ox in active, conscious animals.
The reasons for this are many. First, the sensor must be considered. Systems using neck or throat-fitted optical sensors rely on them being affixed to the animal at the prescribed region while tethered by a cable to the monitor’s controller. The stress of initially applying the sensor in either of these areas causes variations in heart rate and pulse oxygenation and make natural readings unobtainable. Once acclimated to wearing them, the animal must adapt to essentially being at the end of a leash, again, an unnatural condition resulting in skewed readings.
Arguably, placing a conscious animal in an individual animal holder, pacifying and acclimating it to the environment and applying an unobtrusive sensor to its paw is far less disruptive to their normal conditions, and more conducive to acquiring readings that can be relied on.
Remember, any method of restraining your animal will affect the data you’re able to collect. Minimizing the stress of the procedure, reducing the duration of the monitoring period and accelerating how quickly the animal is able to adapt all can have an impact in acquiring more reliable, reproducible readings.
How does the RoVent operate in both constant volume and constant pressure modes?
The RoVent operates in either a constant pressure or a constant volume mode. When in the constant pressure mode it will report the tidal volume delivered. When in the constant volume mode it will report the airway pressure generated to deliver the volume and functions like a standard volume ventilator.
For animal safety the volume mode has both minimum and maximum pressure settings. Regardless of the volume delivered, a minimum airway pressure has to be attained so the animal will be ventilated at a minimal level. Also, regardless of the inspiratory volume, the airway pressure will not go over the maximum pressure setting, thereby protecting the animal from over ventilation. Minimum and maximum pressure are set by the user.
Can I connect an anesthesia mask to my PhysioSuite RoVent?
No, the PhysioSuite RoVent is not designed to ventilate animals using a nose cone. In order to use the PhysioSuite RoVent, the animal must be intubated or tracheotomized.
Is the PhysioSuite compatible with my stereotaxic system?
Yes, the PhysioSuite can be used alongside your stereotaxic frame.
What can I use to clean the warming pads?
They can be cleaned using a germicidal or antiseptic soap with warm water. It is recommended to use a disposable pad cover. This helps to maintain a sterile environment (if necessary) and also aids in the clean-up after the procedure. Because the pads use far infrared heat, there is no concern about the additional layer inhibiting the animal from being warmed properly.
What material are the warming pads?
The pads are comprised of a 3mm carbon wire, a layer of foam insulation, and PVC woven fabric. There is a small metal connector where the wire meets the pad.
Can I sterilize the controller with vaporized hydrogen peroxide (VHP)?
Yes, the controller can be sterilized with hydrogen peroxide gas (or VHP). You may use the same sterilization protocol used for laptop computers and other electronics in your facility. Be sure to open any caps or ports to allow the gas to reach inner mechanisms and tubing, if applicable.