Adult Mouse Brain Matrices, Stainless Steel

Cleaning Instructions

Stainless steel matrices can be autoclaved and/or cold sterilized.

Table 1: Recommended Hard Surface Disinfectants

Name	Example	Comments
Alcohols	70% ethyl alcohol 70% - 99% isopropyl alcohol	Contact time required is 15 minutes Contaminated surfaces take longer to disinfect Remove gross contamination before using Inexpensive Flammable
Quaternary Ammonium	Roccal®, Cetylcide®	Rapidly inactivated by organic matter Compounds may support growth of gram negative bacteria
Chlorine	Sodium hypochlorite (Clorox ® 10% solution) Chlorine dioxide (Clidox®, Alcide®)	Corrosive Presence of organic matter reduces activity Chlorine dioxide must be fresh (<14 days old) Kills vegetative organisms within 3 minutes of contact
Aldehydes	Glutaraldehyde (Cidex®, Cide Wipes®)	Rapidly disinfects surfaces Toxic Exposure limits have been set by OSHA
Phenolics	Lysol®, TBQ®	Less affected by organic material than other disinfectants
Chlorhexidine	Nolvasan®, Hibiclens®	Presence of blood does not interfere with activity Rapidly bactericidal and persistent Effective against many viruses

Table 2: Recommended Instrument Sterilants

Name	Examples	Comments
Physical: Steam Sterilization (Moist Heat)	Autoclave	Effectiveness dependent upon temperature, pressure and time (e.g., 121oC for 15 min. vs 131oC for 3 min)
Dry Heat	Hot Bead Sterilizer, Dry Chamber	Fast. instruments must be cooled before contacting tissue
Ionizing Radiation	Gamma Radiation	Requires special equipment
Chemical: Gas Sterilization	Ethylene Oxide	Requires 30% or greater relative humidity for effectiveness against spores Gas is irritating to tissue; all materials require safe airing time Carcinogenic
Hydrogen Peroxide	(Sterad®)	Not useful for “delicate” items
Chlorine (inst. must be rinsed thoroughly with sterile water)	Chlorine Dioxide (Clidox®, Alcide®)	A minimum of 6 hours required for sterilization Presence of organic matter reduces activity Must be freshly made (<14 days)
Aldehydes (inst. must be rinsed thoroughly with sterile water)	Formaldehyde (6% sol.), Glutaraldehyde	For all aldehydes: Many hours required for sterilization Corrosive and irritating Consult safety representative on proper use Glutaraldehyde is less irritating and less corrosive than formaldehyde

Matrix Dimensions

• A: 3.18 mm
• B: 11.1 mm
• C: 8.73 mm
• D: 19.1 mm
• E: 12.2 mm
• Cavity Depth: 7.4 mm

Reference: The Mouse Brain in Stereotaxic Coordinates

BK-012547637X 
Deluxe 2nd Edition with CD ROM
Author: George Paxinos, Keith B.J. Franklin, Franklin Keith B. J.
Publication date: July 2001 / 296 pages / 2nd Edition

The Mouse Brain in Stereotaxic Coordinates is the most comprehensive and accurate atlas of the mouse brain ever published. The first edition of this book has become the acknowledged reference for the field. For this second edition, the authors have incorporated lower brainstem sections, added an entire sagittal plane section, and revised all delineations, especially of the cortex.

Key features:

• A coronal set (slices are vertical, from front to back) of 100 photographs and 100 matching detailed diagrams delineating the entire brain
• A sagittal set (slices are horizontal, from left to right) featuring 32 photographs and 32 matching detailed diagrams
• Photographs of alternately Nissl- and Acetylcholinesterase-stained sections
• Thoroughly revised cortical delineations and improved subcortical delineations
• All diagrams available on CD-ROM

The CD-ROM contains:

• The entire text of the Atlas
• All images included in the Atlas
• Instructions on editing and printing the images
• Coronal, sagittal, and horizontal color plates